![]() ![]() Multiple beneficial effects of essential oil, such as antioxidation, anti-inflammation, antibacterial, and anticancer activities, have been investigated and applied for prevention and treatment of diseases. The result demonstrated that off-line DPPH-GC-MS coupling CCC is an efficient method for screening, identification, and separation of antioxidant compounds in essential oilĮssential oils are fluid mixtures obtained from fragrant plants, which have been used in cosmetic fragrances, food flavors, and traditional medicines for several centuries. Notably, a significant synergistic effect on DPPH scavenging is found between curzerene and eucalyptol. Camphor, δ-elemene, and β-elemene show weak DPPH scavenging activity, while curzerene and eucalyptol show moderate DPPH scavenging activity. Moreover, these five compounds are isolated by HSCCC using two solvent systems, n-hexane-acetonitrile-ethanol (5:3:2, v/v) and n-hexane-acetonitrile-acetone (4:3:1, v/v), and subjected to DPPH scavenging assay. ![]() ![]() Five compounds are preliminarily screened as antioxidants using DPPH-GC according to the reduction of GC peak areas of each compound after reaction with DPPH and then identified as eucalyptol (7.66%), camphor (2.34%), δ-elemene (1.15%), β-elemene (7.10%), and curzerene (15.77%) using GC-MS. ![]() In the present study, the hyphenation of 2,2′-diphenyl-1-picrylhydrazyl (DPPH)-gas chromatography (GC)-mass spectrometry (MS) offline and high-speed countercurrent chromatography (HSCCC) is established for efficient screening, identification, and isolation of antioxidants from essential oils and applied to the essential oil of Curcuma wenyujin Y.H. Essential oils are an important source of natural antioxidants and multiple methods have been established for evaluation of their overall antioxidant activity, however, the antioxidant activities of their compounds are less investigated. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |